Name : Human IL-2 R alpha/CD25 Protein
Product Source :
Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu22-Cys213.[Accession | P01589]
Molecular Weight :
The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). SPR Data Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay (Biacore T200).
Background :
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Synonyms :
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
References & Citations :
(1)Smith K A. The structure of IL2 bound to the three chains of the IL2 receptor and how signaling occurs[J]. Medical Immunology, 2006, 5(1).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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