Name : Human CD6 Protein
Product Source :
Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His18-Leu402.[Accession | NP_006716.3]
Molecular Weight :
The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD6 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). SPR Data Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 μM as determined in SPR assay (Biacore T200).
Background :
The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
Synonyms :
CD6; TP120; T12
References & Citations :
Simões IT, Aranda F, Casadó-Llombart S, Velasco-de Andrés M, Català C, Álvarez P, Consuegra-Fernández M, Orta-Mascaró M, Merino R, Merino J, Alberola-Ila J, González-Aseguinolaza G, Carreras E, Martínez V, Lozano F. Multifaceted effects of soluble human CD6 in experimental cancer models. J Immunother Cancer. 2020 Mar;8(1):e000172. doi: 10.1136/jitc-2019-000172. PMID: 32217757; PMCID: PMC7174071.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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