Name : Canine BMPR1A/ALK-3 Protein
Product Source :
Recombinant Canine BMPR1A/ALK-3 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln24-Arg152.[Accession | NP_001138622.1]
Molecular Weight :
The protein has a predicted MW of 40.94 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Canine BMPR1A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Canine BMPR1A is greater than 95% as determined by SEC-HPLC. Cell Based Assay Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.5 – 2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
Background :
The type IA bone morphogenetic protein receptor (Bmpr1a), encoded by 11 exons and spanning about 40 kb on chromosome 14 in mice and chromosome 10 in human (Derynck & Feng, 1997; Mishina, Hanks, Miura, Tallquist, & Behringer, 2002), is an essential receptor for BMP signaling.
Synonyms :
BMPR1A; ALK-3; ALK3; ALK 3
References & Citations :
Jing J, Hinton RJ, Feng JQ. Bmpr1a Signaling in Cartilage Development and Endochondral Bone Formation. Vitam Horm. 2015;99:273-91. doi: 10.1016/bs.vh.2015.06.001. Epub 2015 Jul 15. PMID: 26279380.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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