Name : Mouse CD27 Ligand/CD70 Protein
Product Source :
Recombinant Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ser45-Pro195.[Accession | O55237]
Molecular Weight :
The protein has a predicted MW of 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 6.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CD27 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CD27 Ligand is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse CD27, mFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 18.3ng/ml determined by ELISA.
Background :
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Synonyms :
CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G
References & Citations :
(1)Buchan S L , Rogel A , Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy[J]. Blood, 2017:blood-2017-07-741025.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
B7-H4 ProteinMolecular Weight
IL-23 alpha Proteincustom synthesis
Popular categories:
IL-20R beta
CD85j/LIR-1