Name : Mouse CD47 Protein
Product Source :
Recombinant Mouse CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln19-Lys140.[Accession | Q61735-1]
Molecular Weight :
The protein has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse SIRP alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA (QC Test). SPR Data Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).
Background :
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don’t eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Synonyms :
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
References & Citations :
(1) Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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