Name : Mouse CD73/NT5E Protein
Product Source :
Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Trp29-Lys549.[Accession | Q61503]
Molecular Weight :
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA (QC Test). Bioactivity Data Measured by its ability to hydrolyze the 5′-phosphate group from the substrate adenosine-5′-monophosphate (AMP). The specific activity is >10000 pmol/min/μg (QC Test).
Background :
CD73, an ecto-5′-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5′-Nucleotidase activity of CD73 converts extracellular nucleoside‑5’‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Synonyms :
CD73; NT5E; NTE; 5′ nucleotidase (CD73); 5′-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55′-nucleotidase; NT5; NTE; CALJA
References & Citations :
(1)Häusler SF, Del Barrio I M , Diessner J , et al. Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.[J]. Am J Transl Res, 2014, 6(2):129-139.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Integrin alpha 5 beta 1 Proteinsupplier
STAT1 Proteinmedchemexpress
Popular categories:
Siglec-10
IL-20R alpha