Name : Human CD28H/IGPR-1 Protein
Product Source :
Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu23-Gly150.[Accession | Q96BF3-1]
Molecular Weight :
The protein has a predicted MW of 15.1 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD28H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD28H is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD28H, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA.
Background :
CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
Synonyms :
TMIGD2; IGPR1; IGPR-1; CD28H; TPC1
References & Citations :
(1) Zhu Y, Yao S, Iliopoulou BP, Han X, Augustine MM, Xu H, Phennicie RT, Flies SJ, Broadwater M, Ruff W, Taube JM, Zheng L, Luo L, Zhu G, Chen J, Chen L. B7-H5 costimulates human T cells via CD28H. Nat Commun. 2013;4:2043. doi: 10.1038/ncomms3043. PMID: 23784006; PMCID: PMC3698612.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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