Name : Human TPBG/5T4 Protein
Product Source :
Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser32-Ser355.[Accession | Q13641-1]
Molecular Weight :
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TPBG is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TPBG, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Background :
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Synonyms :
M6P1; 5T4AG; WAIF1; TPBG; 5T4
References & Citations :
(1) Harrop R, O’Neill E, Stern PL. Cancer stem cell mobilization and therapeutic targeting of the 5T4 oncofetal antigen. Ther Adv Vaccines Immunother. 2019 Jan 25;7:2515135518821623. doi: 10.1177/2515135518821623. PMID: 30719508; PMCID: PMC6348545.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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