Name : Biotinylated Human CD8 alpha/CD8a Protein
Product Source :
Recombinant Biotinylated Human CD8 alpha/CD8a Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser22-Asp182.[Accession | P01732-1]
Molecular Weight :
The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Biotinylated Human CD8 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Anti-CD8 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA.
Background :
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Synonyms :
CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen
References & Citations :
(1) Gibbings DJ, Marcet-Palacios M, Sekar Y, Ng MC, Befus AD. CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses. BMC Immunol. 2007 Aug 1;8:12. doi: 10.1186/1471-2172-8-12. PMID: 17678538; PMCID: PMC2000912.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CAPN2 ProteinSource
TFRC ProteinStorage & Stability
Popular categories:
IFN-κ
Frizzled-8