Name : Rat CD28 Protein
Product Source :
Recombinant Rat CD28 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Asn20-Leu150.[Accession | P31042]
Molecular Weight :
The protein has a predicted MW of 16.66 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rat CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SPR Data Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 μM as determined in SPR assay (Biacore T200).
Background :
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
References & Citations :
(1)Suntharalingam G , Perry M R , Ward S , et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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