Name : Human CD99/MIC2 Protein
Product Source :
Recombinant Human CD99/MIC2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Asp23-Asp122.[Accession | AAH02584]
Molecular Weight :
The protein has a predicted MW of 36.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD99 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD99 is greater than 95% as determined by SEC-HPLC.
Background :
CD99 is a cell surface protein with unique features and only partly defined mechanisms of action. This molecule is involved in crucial biological processes, including cell adhesion, migration, death, differentiation and diapedesis, and it influences processes associated with inflammation, immune responses and cancer. CD99 is frequently overexpressed in many types of tumors, particularly pediatric tumors including Ewing sarcoma and specific subtypes of leukemia.
Synonyms :
12E7; E2 antigen; MIC2; MIC2X; MIC2Y; CD99; HBA71; MSK5X; 12E7; 1110061M03Rik; 2410026K10Rik; D4; pilr-1; Pilr-l; CD99 molecule
References & Citations :
(1) Pasello M, Manara MC, Scotlandi K. CD99 at the crossroads of physiology and pathology. J Cell Commun Signal. 2018 Mar;12(1):55-68. doi: 10.1007/s12079-017-0445-z. Epub 2018 Jan 6. PMID: 29305692; PMCID: PMC5842202.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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