Name : Human CD300A Protein
Product Source :
Recombinant Human CD300A Protein is expressed from HEK293 with Avi tag at the C-terminus. It contains Leu18-Pro180.[Accession | Q9UGN4-1]
Molecular Weight :
The protein has a predicted MW of 19.46 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD300A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD300A is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD300A, Avi Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 71.9ng/ml determined by ELISA.
Background :
CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
Synonyms :
IGSF12; IRp60; CLM-8; IRC1; CD300a; CLM8; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4
References & Citations :
(1) Karra L, Singh Gangwar R, Shamri R, Puzzovio PG, Cohen-Mor S, Levy BD, Levi-Schaffer F. Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation. J Immunol. 2018 Nov 15;201(10):2998-3005. doi: 10.4049/jimmunol.1801000. Epub 2018 Oct 12. PMID: 30315138.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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