Name : Human AMCase/CHIA Protein
Product Source :
Recombinant Human AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr22-Ala476.[Accession | Q9BZP6-1]
Molecular Weight :
The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human AMCase/CHIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human AMCase/CHIA is greater than 95% as determined by SEC-HPLC.
Background :
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. Despite that AMCase expression in the inflammation is under investigation, little is known regarding its regulation during macrophages’ full maturation and polarization.
Synonyms :
Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL
References & Citations :
(1) Di Rosa M, De Gregorio C, Malaguarnera G, Tuttobene M, Biazzo F, Malaguarnera L. Evaluation of AMCase and CHIT-1 expression in monocyte macrophages lineage. Mol Cell Biochem. 2013 Feb;374(1-2):73-80. doi: 10.1007/s11010-012-1506-5. Epub 2012 Nov 6. PMID: 23129258.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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