Name : Biotinylated Mouse CDCP1 Protein
Product Source :
Recombinant Biotinylated Mouse CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Ala667.[Accession | Q5U462]
Molecular Weight :
The protein has a predicted MW of 75.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse CDCP1 is greater than 95% as determined by SEC-HPLC. SPR Data Anti-CDCP1 Antibody captured on CM5 Chip via Protein A can bind Biotinylated Mouse CDCP-1, His Tag with an affinity constant of 0.45 μM as determined in SPR assay (Biacore T200).
Background :
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Synonyms :
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
References & Citations :
(1)Wright HJ, Hou J, Xu B, Cortez M, Potma EO, Tromberg BJ, Razorenova OV. CDCP1 drives triple-negative breast cancer metastasis through reduction of lipid-droplet abundance and stimulation of fatty acid oxidation. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6556-E6565. doi: 10.1073/pnas.1703791114. Epub 2017 Jul 24. PMID: 28739932; PMCID: PMC5559020.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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