Name : Cynomolgus CD3e/CD3 epsilon Protein
Product Source :
Recombinant Cynomolgus CD3e/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln22-Asp117.[Accession | Q95LI5-1]
Molecular Weight :
The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CD3e on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CD3e is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CD3e, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3e Antibody, hFc Tag with the EC50 of 78.8ng/ml determined by ELISA.
Background :
CD3e, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Synonyms :
CD3e; CD3e; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
References & Citations :
(1)Karasu Ebru E, Nilsson Bo B, Köhl Jörg J, Lambris John D JD
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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