Name : Human CD3g/CD3 gamma Protein

Product Source :
Recombinant Human CD3g/CD3 gamma Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gln23-Ser116.[Accession | P09693]

Molecular Weight :
The protein has a predicted MW of 12.31 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CD3g on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD3g is greater than 95% as determined by SEC-HPLC.

Background :
CD3 gamma, a subunit of the T cell receptor-CD3 (TCR/CD3) complex, helps to support surface TCR/CD3 expression and participates in signal transduction for gene induction after antigen recognition by T lymphocytes, and in TCR/CD3 down-modulation.

Synonyms :
CD3g; T3G; CD3 gamma; IMD17

References & Citations :
Sun JY, Pacheco-Castro A, Borroto A, Alarcon B, Alvarez-Zapata D, Regueiro JR. Construction of retroviral vectors carrying human CD3 gamma cDNA and reconstitution of CD3 gamma expression and T cell receptor surface expression and function in a CD3 gamma-deficient mutant T cell line. Hum Gene Ther. 1997 Jun 10;8(9):1041-8. doi: 10.1089/hum.1997.8.9-1041. PMID: 9189762.

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