Name : Human CDH6/Cadherin-6 Protein

Product Source :
Recombinant Human CDH6/Cadherin-6 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser54-Ala615.[Accession | P55285-1]

Molecular Weight :
The protein has a predicted MW of 63.62 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 90% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CDH6 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. ELISA Data Immobilized Human CDH6, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 76.8ng/ml determined by ELISA.

Background :
Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity.

Synonyms :
Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD;

References & Citations :
Zhou W, Santos L, Dimitriadis E. Characterization of the role for cadherin 6 in the regulation of human endometrial receptivity. Reprod Biol Endocrinol. 2020 Jun 29;18(1):66. doi: 10.1186/s12958-020-00624-w. PMID: 32600462; PMCID: PMC7322878.

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