Name : Human E-Cadherin/Cadherin-1 Protein
Product Source :
Recombinant Human E-Cadherin/Cadherin-1 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Asp155-Ala709.[Accession | P12830-1]
Molecular Weight :
The protein has a predicted MW of 61.62 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human E-Cadherin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human E-Cadherin is greater than 90% as determined by SEC-HPLC.
Background :
E-cadherin is the core component of epithelial adherens junctions, essential for tissue development, differentiation, and maintenance. It is also fundamental for tissue barrier formation, a critical function of epithelial tissues.
Synonyms :
Cadherin-1; Cadherin1; E-Cadherin; E Cadherin; Epithelial cadherin; CDH1; CD324; Arc-1; CAD1; ECAD; E-CAD; LCAM; UVO; L-CAM;
References & Citations :
Daulagala AC, Bridges MC, Kourtidis A. E-cadherin Beyond Structure: A Signaling Hub in Colon Homeostasis and Disease. Int J Mol Sci. 2019 Jun 5;20(11):2756. doi: 10.3390/ijms20112756. PMID: 31195621; PMCID: PMC6600153.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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