Name : Human Complement factor I Protein
Product Source :
Recombinant Human Complement factor I Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys19-Val583.[Accession | P05156]
Molecular Weight :
The protein has a predicted MW of 64.58 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 75-95 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Complement factor I on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Complement factor I is greater than 95% as determined by SEC-HPLC.
Background :
Complement factor I (CFI) is a serine protease which plays a key role in the modulation of complement system and the induced-fit factor responsible for controlling the complement-mediated processes.
Synonyms :
EC:3.4.21.45; C3B/C4B inactivator; CFI; IF; C3BINA; FIC3b-INA; I factor (complement); KAF
References & Citations :
Lv W, Ma A, Chi X, Li Q, Pang Y, Su P. A novel complement factor I involving in the complement system immune response from Lampetra morii. Fish Shellfish Immunol. 2020 Mar;98:988-994. doi: 10.1016/j.fsi.2019.11.017. Epub 2019 Nov 8. PMID: 31712129.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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