Name : Rat Coagulation Factor II Protein
Product Source :
Recombinant Rat Coagulation Factor II Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gln25-Arg617.[Accession | NP_075213.2]
Molecular Weight :
The protein has a predicted MW of 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rat Coagulation Factor II on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Rat Coagulation Factor II is greater than 95% as determined by SEC-HPLC.
Background :
Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.
Synonyms :
F2; Prothrombin; thrombin; F II; FII
References & Citations :
Acquasaliente L, Pelc LA, Di Cera E. Probing prothrombin structure by limited proteolysis. Sci Rep. 2019 Apr 16;9(1):6125. doi: 10.1038/s41598-019-42524-z. PMID: 30992526; PMCID: PMC6467981.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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