Name : Human CLEC-1 Protein
Product Source :
Recombinant Human CLEC-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Gln74-Asp280.[Accession | Q8NC01]
Molecular Weight :
The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CLEC-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human CLEC-1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA.
Background :
C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1β production in response to A. fumigatus keratitis.
Synonyms :
CLEC-1; CLEC1; CLEC1A; MGC34328
References & Citations :
(1)Zhu G, Lyu L, Yang H, Lee J, Sun J, Zhang J, Xue S, Yan H, Wang L, Chen X, Che C. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis. Invest Ophthalmol Vis Sci. 2021 May 3;62(6):28. doi: 10.1167/iovs.62.6.28. PMID: 34043748; PMCID: PMC8164365.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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