Name : Human CLEC4A/DCIR Protein
Product Source :
Recombinant Human CLEC4A/DCIR Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln70-Leu237.[Accession | Q9UMR7-1]
Molecular Weight :
The protein has a predicted MW of 46.7 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CLEC4A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CLEC4A is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CLEC4A, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC4A Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA.
Background :
Clec4a has been reported to be an immune suppressor of dendritic cells (DCs), but its potential role in cancer therapy remains to be elucidated. silencing of Clec4a2 expression via skin delivery of shRNA produces an effective antitumor response and that Clec4a2 shRNA may have therapeutic potential as an adjuvant for cancer immunotherapy.
Synonyms :
CD367; Clec4a2; CLECSF6; DCIR; DDB27; HDCGC13P; LLIR; CLEC4A; DCIRLLIR
References & Citations :
(1) Weng TY, Li CJ, Li CY, Hung YH, Yen MC, Chang YW, Chen YH, Chen YL, Hsu HP, Chang JY, Lai MD. Skin Delivery of Clec4a Small Hairpin RNA Elicited an Effective Antitumor Response by Enhancing CD8 Immunity In Vivo. Mol Ther Nucleic Acids. 2017 Dec 15;9:419-427. doi: 10.1016/j.omtn.2017.10.015. Epub 2017 Oct 26. PMID: 29246320; PMCID: PMC5701806.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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