Name : Human MDL-1/CLEC5A Protein
Product Source :
Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Tyr26-Lys188.[Accession | Q9NY25-1]
Molecular Weight :
The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MDL-1, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
Background :
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
Synonyms :
MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
References & Citations :
(1) Riesen M, Feyst I, Rattanavirotkul N, Ezcurra M, Tullet JM, Papatheodorou I, Ziehm M, Au C, Gilliat AF, Hellberg J, Thornton JM, Gems D. MDL-1, a growth- and tumor-suppressor, slows aging and prevents germline hyperplasia and hypertrophy in C. elegans. Aging (Albany NY). 2014 Feb;6(2):98-117. doi: 10.18632/aging.100638. PMID: 24531613; PMCID: PMC3969279.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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