Name : Mouse CLEC14A Protein

Product Source :
Recombinant Mouse CLEC14A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu22-Thr386.[Accession | Q8VCP9]

Molecular Weight :
The protein has a predicted MW of 40.33 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse CLEC14A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CLEC14A is greater than 95% as determined by SEC-HPLC.

Background :
CLEC14A is a single-pass transmembrane glycoprotein belonging to the vascular expressed C-type lectin family. CLEC14A is found to be expressed in vascular endothelial cells during embryogenesis and is also implicated in tumor angiogenesis.

Synonyms :
Epidermal growth factor receptor 5; EGFR-5; EGFR 5; EGFR5; C14orf27; UNQ236; PRO269; CEG1

References & Citations :
Su Z, Li Y, Lv H, Cui X, Liu M, Wang Z, Zhang Y, Zhen J, Tang W, Wang X, Yi F. CLEC14A protects against podocyte injury in mice with adriamycin nephropathy. FASEB J. 2021 Jul;35(7):e21711. doi: 10.1096/fj.202100283R. PMID: 34107098.

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