Name : Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein
Product Source :
Recombinant Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr260-His493.[Accession | Q9UKU9-1]
Molecular Weight :
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human ANGPTL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human ANGPTL2 is greater than 95% as determined by SEC-HPLC. SPR Data Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human ANGPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay (Biacore T200).
Background :
Angiopoietin-like 2 (ANGPTL2) is a proinflammatory protein belonging to the angiopoietin-like family. ANGPTL2 is secreted and detected in the systemic circulation. Different observational clinical studies reported that circulating levels of ANGPTL2 increase significantly in various chronic inflammatory diseases and showed associations between ANGPTL2 levels and diagnosis and/or prognosis of cardiovascular diseases, diabetes, chronic kidney disease, and various types of cancers.
Synonyms :
ARP2; HARP; angiopoietin-like 2; ANGPTL2; ANGRP2; MGC8889
References & Citations :
(1) Thorin-Trescases N, Thorin E. High Circulating Levels of ANGPTL2: Beyond a Clinical Marker of Systemic Inflammation. Oxid Med Cell Longev. 2017;2017:1096385. doi: 10.1155/2017/1096385. Epub 2017 Aug 24. PMID: 29138671; PMCID: PMC5613648.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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