Name : Human CD13/ANPEP Protein
Product Source :
Recombinant Human CD13/ANPEP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys69-Lys967.[Accession | P15144]
Molecular Weight :
The protein has a predicted MW of 103.8 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD13/ANPEP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD13/ANPEP is greater than 95% as determined by SEC-HPLC.
Background :
CD13/aminopeptidase N is a widely expressed ectoenzyme with multiple functions. As an enzyme, CD13 regulates activities of numerous cytokines by cleaving their N-terminals and is involved in Ag processing by trimming the peptides bound to MHC class II. Independent of its enzymatic activity, cell membrane CD13 functions by cross-linking-induced signal transduction, regulation of receptor recycling, enhancement of FcγR-mediated phagocytosis, and acting as a receptor for cytokines.
Synonyms :
AP-N; AP-M; ANPEP; APN; CD13; PEPN; Cd13; CD13APN; gp150; LAP1; PEPNhAPN
References & Citations :
(1) Lu C, Amin MA, Fox DA. CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders. J Immunol. 2020 Jan 1;204(1):3-11. doi: 10.4049/jimmunol.1900868. PMID: 31848300; PMCID: PMC6997018.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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