Name : SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein
Product Source :
Recombinant SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg319-Phe541(L452R, T478K).[Accession | QHD43416.1]
Molecular Weight :
The protein has a predicted MW of 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE SARS-COV-2 Spike RBD (Delta B.1.617.2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of SARS-COV-2 Spike RBD (Delta B.1.617.2) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
Background :
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.
Synonyms :
S protein RBD; S glycoprotein RBD; Spike protein RBD
References & Citations :
(1)Gu H, Chen Q, Yang G, He L, Fan H, Deng YQ, Wang Y, Teng Y, Zhao Z, Cui Y, Li Y, Li XF, Li J, Zhang NN, Yang X, Chen S, Guo Y, Zhao G, Wang X, Luo DY, Wang H, Yang X, Li Y, Han G, He Y, Zhou X, Geng S, Sheng X, Jiang S, Sun S, Qin CF, Zhou Y. Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy. Science. 2020 Sep 25;369(6511):1603-1607. doi: 10.1126/science.abc4730. Epub 2020 Jul 30. PMID: 32732280; PMCID: PMC7574913.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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