Name : Cynomolgus/Rhesus macaque DKK1 Protein
Product Source :
Recombinant Cynomolgus/Rhesus macaque DKK1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Thr32-His266.[Accession | XP_005565907.1 / NP_001247454.1]
Molecular Weight :
The protein has a predicted MW of 51.42 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus/Rhesus macaque DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus/Rhesus macaque DKK1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
Background :
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Synonyms :
Dickkopf-1; Dkk1; SK
References & Citations :
(1)Zhang W , Drake M T. Potential Role for Therapies Targeting DKK1, LRP5, and Serotonin in the Treatment of Osteoporosis[J]. Current Osteoporosis Reports, 2012, 10(1):93-100.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
NXPH1 Proteinweb
CDH16 Proteinmanufacturer
Popular categories:
Vitamin D Receptor
SRSF Protein Kinase 1