Name : Biotinylated Human DKK3 Protein
Product Source :
Recombinant Biotinylated Human DKK3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro23-Ile350.[Accession | Q9UBP4]
Molecular Weight :
The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human DKK3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human DKK3 is greater than 95% as determined by SEC-HPLC.
Background :
Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is one of four numbered members of the Dickkopf family of Wnt antagonists.DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Synonyms :
Dickkopf-3; Dkk3; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1; AW061014; C87148
References & Citations :
(1) Guo Q , Qin W. DKK3 blocked translocation of β-catenin/EMT induced by hypoxia and improved gemcitabine therapeutic effect in pancreatic cancer Bxpc-3 cell[J]. Journal of Cellular and Molecular Medicine, 2015:n/a-n/a.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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