Name : Cynomolgus DLK1 Protein

Product Source :
Recombinant Cynomolgus DLK1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala24-Cys306.[Accession | XP_005562275.1]

Molecular Weight :
The protein has a predicted MW of 31.26 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus DLK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus DLK1 is greater than 95% as determined by SEC-HPLC.

Background :
paternally inherited genetic defects of DLK1 were identified in four families with nonsyndromic CPP and a metabolic phenotype. DLK1 encodes a transmembrane protein that is important for adipose tissue homeostasis and neurogenesis and is located in the imprinted chromosome 14q32 region associated with Temple syndrome.

Synonyms :
pG2; FA1; DLK; DLK1; DLK-1; Pref1; secredeltin; ZOG

References & Citations :
(1) Macedo DB, Kaiser UB. DLK1, Notch Signaling and the Timing of Puberty. Semin Reprod Med. 2019 Jul;37(4):174-181. doi: 10.1055/s-0039-3400963. Epub 2020 Jan 23. PMID: 31972862.

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