Name : Cynomolgus ANXA1 Protein

Product Source :
Recombinant Cynomolgus ANXA1 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Ala2-Asn346.[Accession | XP_005581995.1]

Molecular Weight :
The protein has a predicted MW of 39.69 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus ANXA1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus ANXA1 is greater than 95% as determined by SEC-HPLC.

Background :
Atherosclerosis, characterized by the formation of fat-laden plaques, is a chronic inflammatory disease. ABCA1 promotes cholesterol efflux, reduces cellular cholesterol accumulation, and regulates anti-inflammatory activities in an apoA-I- or ANXA1-dependent manner. The latter activity occurs by mediating the efflux of ANXA1, which plays a critical role in anti-inflammatory effects, cholesterol transport, exosome and microparticle secretion, and apoptotic cell clearance.

Synonyms :
Annexin I; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; p35; ANX1; LPC1; Annexin A1; Annexin-1; ANXA1

References & Citations :
(1) Shen X, Zhang S, Guo Z, Xing D, Chen W. The crosstalk of ABCA1 and ANXA1: a potential mechanism for protection against atherosclerosis. Mol Med. 2020 Sep 7;26(1):84. doi: 10.1186/s10020-020-00213-y. PMID: 32894039; PMCID: PMC7487582.

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