Name : Human DPPIV/CD26 Protein
Product Source :
Recombinant Human DPPIV/CD26 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asn29-Pro766.[Accession | P27487]
Molecular Weight :
The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 91-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human DPPIV on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human DPPIV is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human DPPIV, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).. Bioactivity Data Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg (QC Test).
Background :
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.
Synonyms :
ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5
References & Citations :
(1)Enz N, Vliegen G, De Meester I, Jungraithmayr W. DPPIV/CD26 – a potential biomarker and target for cancer therapy. Pharmacol Ther. 2019 Jun;198:135-159. doi: 10.1016/j.pharmthera.2019.02.015. Epub 2019 Feb 26. PMID: 30822465.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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