Name : Human DR6/TNFRSF21 Protein
Product Source :
Recombinant Human DR6/TNFRSF21 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln42-His349.[Accession | O75509]
Molecular Weight :
The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human DR6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human DR6, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DR6 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Background :
beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. DR6 is broadly expressed by developing neurons, and is required for normal cell body death and axonal pruning both in vivo and after trophic-factor deprivation in vitro.DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation.
Synonyms :
Death receptor 6; CD358; TNFRSF21; DR6; BM-018; MGC31965
References & Citations :
(1) Nikolaev A, McLaughlin T, O’Leary DD, Tessier-Lavigne M. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature. 2009 Feb 19;457(7232):981-9. doi: 10.1038/nature07767. PMID: 19225519; PMCID: PMC2677572.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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