Name : Human ANXA2 Protein
Product Source :
Recombinant Human ANXA2 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser2-Asp339.[Accession | P07355]
Molecular Weight :
The protein has a predicted MW of 39.6 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ANXA2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.
Synonyms :
Chromobindin-8; Lipocortin II; PAP-IV; ANX2; ANX2L4; CAL1H; LPC2D; ANXA2; Annexin A2; Annexin II; LIP2PAP-IV; LPC2D; LPC2DP36; p36
References & Citations :
(1) Zhang Y, Bi J, Zhu H, Shi M, Zeng X. ANXA2 could act as a moderator of EGFR-directed therapy resistance in triple negative breast cancer. Biosci Biotechnol Biochem. 2018 Oct;82(10):1733-1741. doi: 10.1080/09168451.2018.1484275. Epub 2018 Jun 18. PMID: 29912633.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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