Name : Human EDA2R Protein

Product Source :
Recombinant Human EDA2R Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met1-Thr138.[Accession | Q9HAV5-1]

Molecular Weight :
The protein has a predicted MW of 42.08 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human EDA2R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human EDA2R is greater than 95% as determined by SEC-HPLC.

Background :
The X-linked ectodermal dysplasia receptor (XEDAR; also known as EDA2R or TNFRSF27) is a member of the TNFR superfamily that is highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2), a member of the TNF family that is encoded by the anhidrotic ectodermal dysplasia (EDA) gene.

Synonyms :
X-linked ectodysplasin-A2 receptor; EDA-A2 receptor; EDA-A2; Tnfrsf27; Xedar

References & Citations :
Verhelst K, Gardam S, Borghi A, Kreike M, Carpentier I, Beyaert R. XEDAR activates the non-canonical NF-κB pathway. Biochem Biophys Res Commun. 2015 Sep 18;465(2):275-80. doi: 10.1016/j.bbrc.2015.08.019. Epub 2015 Aug 8. PMID: 26260321.

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