Name : Human EMAP-II/AIMP1 Protein

Product Source :
Recombinant Human EMAP-II/AIMP1 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ala2-Lys312.[Accession | Q12904-1]

Molecular Weight :
The protein has a predicted MW of 35.4 kDa. The protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 8.0).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human EMAP-II on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human EMAP-II, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EMAP-II Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA.

Background :
The potent antiangiogenic and proinflammatory protein Endothelial Monocyte Activating Polypeptide II (EMAP II) has been described as a mediator of pulmonary vascular and alveolar formation and its expression is inversely related to the periods of vascularization and alveolarization in the developing lung. Hence the study of EMAP II could play a vital role in studying and devising appropriate therapeutics for diseases of aberrant lung development, such as BPD.

Synonyms :
EMAP-2; EMAP-II; AIMP1; HLD3; SCYE1; p43

References & Citations :
(1) Lal CV, Schwarz MA. Vascular mediators in chronic lung disease of infancy: role of endothelial monocyte activating polypeptide II (EMAP II). Birth Defects Res A Clin Mol Teratol. 2014 Mar;100(3):180-8. doi: 10.1002/bdra.23234. Epub 2014 Mar 12. PMID: 24619875; PMCID: PMC4280095.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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