Name : Monkeypox virus E8L Protein
Product Source :
Recombinant Monkeypox virus E8L Protein is expressed from E.coli with His tag at the N-Terminus. It contains Met1-Thr275.[Accession | QJQ40248.1]
Molecular Weight :
The protein has a predicted MW of 32.92 kDa. The protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Monkeypox virus E8L on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells.
Synonyms :
Cell surface-binding protein; Carbonic anhydrase homolog
References & Citations :
(1) Shchelkunov SN, Totmenin AV, Safronov PF, Mikheev MV, Gutorov VV, Ryazankina OI, Petrov NA, Babkin IV, Uvarova EA, Sandakhchiev LS, Sisler JR, Esposito JJ, Damon IK, Jahrling PB, Moss B. Analysis of the monkeypox virus genome. Virology. 2002 Jun 5;297(2):172-94. doi: 10.1006/viro.2002.1446. PMID: 12083817.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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