Name : Mouse ECSCR Protein
Product Source :
Recombinant Mouse ECSCR Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln19-Thr130.[Accession | Q3TZW0-1]
Molecular Weight :
The protein has a predicted MW of 38.6 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris,150mM NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse ECSCR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse ECSCR is greater than 95% as determined by SEC-HPLC.
Background :
Endothelial cell-specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF).
Synonyms :
ARIA; ECSCR; ECSM2
References & Citations :
(1) Verma A, Bhattacharya R, Remadevi I, Li K, Pramanik K, Samant GV, Horswill M, Chun CZ, Zhao B, Wang E, Miao RQ, Mukhopadhyay D, Ramchandran R, Wilkinson GA. Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. Blood. 2010 Jun 3;115(22):4614-22. doi: 10.1182/blood-2009-10-248856. Epub 2010 Jan 19. PMID: 20086248; PMCID: PMC2881497.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
ITIH5 ProteinFormulation
FKBP12 ProteinPurity & Documentation
Popular categories:
TGF-beta Superfamily
Calcineurin B