Name : Human/Cynomolgus EFNA3/Ephrin A3 Protein
Product Source :
Recombinant Human/Cynomolgus EFNA3/Ephrin A3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln23-Ser213.[Accession | P52797-1]
Molecular Weight :
The protein has a predicted MW of 22.51 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human/Cynomolgus EFNA3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human/Cynomolgus EFNA3 is greater than 95% as determined by SEC-HPLC.
Background :
Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.
Synonyms :
Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3
References & Citations :
(1) Duffy SL, Steiner KA, Tam PP, Boyd AW. Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Gene Expr Patterns. 2006 Oct;6(7):719-23. doi: 10.1016/j.modgep.2005.12.007. Epub 2006 Feb 8. PMID: 16466970.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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