Name : Human EFEMP1 Protein
Product Source :
Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Gln18-Phe493.[Accession | Q12805-1]
Molecular Weight :
The protein has a predicted MW of 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EFEMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human EFEMP1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA.
Background :
Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.
Synonyms :
EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5
References & Citations :
(1) Peng X, Guo Z, Zhang Y, Sun B, Zhang Q. EFEMP1 in Direct Inguinal Hernia: correlation with TIMP3 and Regulation Toward Elastin Homoeostasis as Well as Fibroblast Mobility. J Invest Surg. 2020 Nov 1:1-9. doi: 10.1080/08941939.2020.1811812. Epub ahead of print. Erratum in: J Invest Surg. 2020 Sep 22;:1-2. PMID: 33131351.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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