Name : Human Endoglin/CD105 Protein
Product Source :
Recombinant Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu26-Gly586.[Accession | P17813-1]
Molecular Weight :
The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Endoglin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human Endoglin is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Endoglin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Background :
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.
Synonyms :
CD105; Endoglin; ENG; ORW; ORW1; END; HHT1
References & Citations :
(1)Rosen L S , Gordon M S , Robert F , et al. Endoglin for Targeted Cancer Treatment[J]. Current Oncology Reports, 2014, 16(2):365-482.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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