Name : Mouse EDA2R Protein
Product Source :
Recombinant Mouse EDA2R Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met1-Thr138.[Accession | Q8BX35]
Molecular Weight :
The protein has a predicted MW of 42.15 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse EDA2R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse EDA2R is greater than 95% as determined by SEC-HPLC.
Background :
EDA2R is a member of the large family of tumor necrosis factor receptor (TNFR). Previous studies suggested that EDA2R expression might be increased in the kidneys of diabetic mice. High glucose increases EDA2R expression in kidney cells and that EDA2R induces podocyte apoptosis and dedifferentiation in high glucose milieu partially through enhanced ROS generation.
Synonyms :
X-linked ectodysplasin-A2 receptor; EDA-A2 receptor; EDA-A2; Tnfrsf27; Xedar
References & Citations :
Lan X, Kumar V, Jha A, Aslam R, Wang H, Chen K, Yu Y, He W, Chen F, Luo H, Malhotra A, Singhal PC. EDA2R mediates podocyte injury in high glucose milieu. Biochimie. 2020 Jul;174:74-83. doi: 10.1016/j.biochi.2020.04.003. Epub 2020 Apr 15. PMID: 32304771; PMCID: PMC7282945.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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