Name : Human EGFR/HER1 Protein
Product Source :
Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu25-Ser645.[Accession | P00533]
Molecular Weight :
The protein has a predicted MW of 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EGFR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human EGFR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human EGF, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 2.4μg/ml determined by ELISA.
Background :
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Synonyms :
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
References & Citations :
(1)Seshacharyulu P , Ponnusamy M P , Haridas D , et al. Targeting the EGFR signaling pathway in cancer therapy[J]. Expert Opinion on Therapeutic Targets, 2012.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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