Name : Human ENPP-1 Protein
Product Source :
Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Lys98-Asp925.[Accession | P22413]
Molecular Weight :
The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ENPP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human ENPP-1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA. Bioactivity Data Measured by its ability to hydrolyze thymidine 5′-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/μg, as measured under the described conditions.
Background :
Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Synonyms :
E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
References & Citations :
(1) Xu HG, Li ZR, Wang H, Liu P, Xiang SN, Wang CD, Zhang XL. Expression of ectonucleotide pyrophosphatase-1 in end-plate chondrocytes with transforming growth factor beta 1 siRNA interference by cyclic mechanical tension. Chin Med J (Engl). 2013 Oct;126(20):3886-90. PMID: 24157151.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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