Name : Human ENPP-2/Autotaxin Protein
Product Source :
Recombinant Human ENPP-2/Autotaxin Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Asp49-Ile863.[Accession | NP_001035181]
Molecular Weight :
The protein has a predicted MW of 94.8 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ENPP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ENPP-2 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/μg, as measured under the described conditions.
Background :
Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.
Synonyms :
PDNP2; ATX-X; NPP2; PD-IALPHA; ENPP-2; E-NPP 2; Autotaxin; LysoPLD; ATX
References & Citations :
(1)Srivastava D, Kukkuta Sarma GR, Dsouza DS, Muralidharan M, Srinivasan K, Mandal AK. Characterization of residue-specific glutathionylation of CSF proteins in multiple sclerosis – A MS-based approach. Anal Biochem. 2019;564-565:108-115. doi:10.1016/j.ab.2018.10.015
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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