Name : Human Ephrin-B2/EFNB2 Protein
Product Source :
Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile28-Ala229.[Accession | NP_004084.1]
Molecular Weight :
The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Ephrin B2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human EPHB2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). SPR Data Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Background :
Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
Synonyms :
Ephrin B2; EPLG5; HTKL; LERK5; EFNB2
References & Citations :
(1) Nakayama A, Nakayama M, Turner CJ, Höing S, Lepore JJ, Adams RH. Ephrin-B2 controls PDGFRβ internalization and signaling. Genes Dev. 2013 Dec 1;27(23):2576-89. doi: 10.1101/gad.224089.113. PMID: 24298057; PMCID: PMC3861671.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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