Name : Human Ephrin-A4/EFNA4 Protein
Product Source :
Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu26-Gly171.[Accession | P52798-1]
Molecular Weight :
The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Ephrin-A4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC. SPR Data Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 μM as determined in SPR assay (Biacore T200).
Background :
Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.
Synonyms :
Ephrin-A4; Ephrin A4; LERK-4; EFNA4; EPLG4; EFL4
References & Citations :
(1) Abdou AG, Abd el-Wahed MM, Asaad NY, Samaka RM, Abdallaha R. Ephrin A4 expression in osteosarcoma, impact on prognosis, and patient outcome. Indian J Cancer. 2010 Jan-Mar;47(1):46-52. doi: 10.4103/0019-509X.58859. PMID: 20071790.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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