Name : Biotinylated Cynomolgus EPHA2 Protein
Product Source :
Recombinant Biotinylated Cynomolgus EPHA2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala24-Ser534.[Accession | Q1KL86]
Molecular Weight :
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Cynomolgus EPHA2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Cynomolgus EPHA2 is greater than 95% as determined by SEC-HPLC.
Background :
Ephrin type-A receptor 2 (EPHA2) is a receptor tyrosine kinase (RTK), whose over-expression has been observed in a variety of cancers, including breast cancer. EPHA2 expression may be causally related to tumorigenesis; therefore, it is important to understand how EPHA2 gene (EPHA2) expression is regulated.
Synonyms :
Ephrin type-A receptor 2; EPHA2
References & Citations :
Okuyama T, Sakamoto R, Kumagai K, Nishizawa M, Kimura T, Sugie T, Kimura T. EPHA2 antisense RNA modulates EPHA2 mRNA levels in basal-like/triple-negative breast cancer cells. Biochimie. 2020 Dec;179:169-180. doi: 10.1016/j.biochi.2020.10.002. Epub 2020 Oct 3. PMID: 33022313.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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