Name : Cynomolgus EphB2 Protein
Product Source :
Recombinant Cynomolgus EphB2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val19-Pro542.[Accession | A0A7N9CQH5]
Molecular Weight :
The protein has a predicted MW of 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus EphB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus EphB2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus EphB2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EphB2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Background :
EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EphB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EphB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EphB2 stabilization by hypoxia.
Synonyms :
DRT; ERK; HEK5; TYRO5; CAPB; EK5; EPHT3; PCBC; Cek5; EphB2; EPHT3MGC87492; Nuk; Qek2; Sek3
References & Citations :
(1) Qiu W, Song S, Chen W, Zhang J, Yang H, Chen Y. Hypoxia-induced EphB2 promotes invasive potential of glioblastoma. Int J Clin Exp Pathol. 2019 Feb 1;12(2):539-548. PMID: 31933858; PMCID: PMC6945092.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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