Name : Human EphA4 Protein
Product Source :
Recombinant Human EphA4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val20-Thr547.[Accession | P54764-1]
Molecular Weight :
The protein has a predicted MW of 59.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EphA4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human EphA4 is greater than 95% as determined by SEC-HPLC.
Background :
The expression and activation of EphA4 in the various cell types in a knee joint was upregulated upon an intraarticular injury. To determine if EphA4 signaling plays a role in osteoarthritis, we determined whether deficient EphA4 expression (in EphA4 knockout mice) or upregulation of the EphA4 signaling (with the EfnA4-fc treatment) would alter cellular functions of synoviocytes and articular chondrocytes. In synoviocytes, deficient EphA4 expression enhanced, whereas activation of the EphA4 signaling reduced, expression and secretion of key inflammatory cytokines and matrix metalloproteases.
Synonyms :
Sek; Sek1; EphA4; HEK8; SEK; TYRO1; EK8
References & Citations :
(1) Stiffel VM, Thomas A, Rundle CH, Sheng MH, Lau KW. The EphA4 Signaling is Anti-catabolic in Synoviocytes but Pro-anabolic in Articular Chondrocytes. Calcif Tissue Int. 2020 Dec;107(6):576-592. doi: 10.1007/s00223-020-00747-7. Epub 2020 Aug 20. PMID: 32816052; PMCID: PMC7606366.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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